NADPH in kits

NADPH is used in the fluorescence kits from IMCO for assay of Trx, Grx or TrxR. Solutions of NADPH are only stable for about 10 days even if stored frozen.

You can make a fresh solution by dissolving 2 mg of NADPH in 0.5 ml of buffer. A solution of NADPH which is turning yellow should be discarded. By making fresh NADPH your assay will work for a long time. Other components of the kits are stable if stored frozen or according to the kit instructions.


New kit for high sensitivity GSH measurements

Measuring GSH in plasma and serum requires high sensitivity and is complicated by oxidation in samples upon storage. IMCO has developed a new kit for high sensitivity fluorescent determinations of GSH, GSSG and glutathionylated products.


Mammalian thioredoxin reductases: TR-03 and TR-03B

Mammalian thioredoxin reductase 1 (TrxR) contain an essential seleocycteine residue encoded by the stop codon UGA. This makes recombinant expression in E.coli a challenge. IMCO now has preparations of rat TrxR with unsurpassed specific activity.