Glutathionylation and GRX activity easy to study
Glutathionylation (the formation of a mixed disulfide with GSH)can be studied by products from IMCO. We also have a new unique kit for assays of glutaredoxin activity.
IMCO has DiEGSSG and GRX-04 as a products for studying glutathionylation. DiEGSSG or dieosine-diglutathione disulfide is fluoresent and can be used to label a protein┤s SH-group. The glutathionylated protein is visible in ultraviolet light on gelelectrophoresis and can be specifically deglutahionylated by E. coli GRX1C14S mutant protein ( GRX-04) in the presence of 1mM GSH and glutathione reductase plus NADPH. The reaction can be followed by the high fluorescence of released E-GSH(eosine-glutathione).
For easy and highly sensitive measurements of glutaredoxin activity use FkGRX-01 containing all components for pmol sensitivity in microtiter plate assays in 100 Ál volumes. Determine eg Grx activiy in 40 Ál plasma using the kit. Grx1 activity is a novel marker of oxidative stress.
FkGRX-01 (pdf, 164 kB)