New Product and Price list
Imco has a new kit for high sensitivity measurements of GSH using a glutaredoxin-based method and fluorescent dieosin-GSSG.
FkGSH-01 Kit for high sensitivity GSH measurements
Measuring GSH in plasma and serum requires high sensitivity and is complicated by oxidation in samples upon storage. IMCO has developed a new kit for high sensitivity fluorescent determinations of GSH, GSSG and glutathionylated products.
The new kit is now launched and is sensitive in the range 0-1.0 ÁM which means that 20 or 40 Ál of plasma or serum can be used.
Mammalian thioredoxin reductases: TR-03 and TR-03B
Mammalian thioredoxin reductase 1 (TrxR) contain an essential seleocycteine residue encoded by the stop codon UGA. This makes recombinant expression in E.coli a challenge. IMCO now has preparations of rat TrxR with unsurpassed specific activity.
The rat TR-03 and TR-03B enzymes show full cross-reactivity with all mammalian thioredoxins as well as other Trx like E.coli Trx1.
For reference see:
Cheng, Q and Arner ESJ : Selenocysteine insertion at a predefined UAG codon in a release factor 1 (RF1)-depleted Escherichia coli host strain bypasses species barriers in recombinant selenoprotein translation. (2017), J. Biol. Chem 292, 5476-5487.
JBC article (pdf, 3086 kB)